Igg1 Isotype Control Antibody

Reafinity antibodies have been engineered for their high specificity and contain human igg1 parts for constant regions.

Igg1 isotype control antibody.

Immunoglobulin g igg is a type of antibody representing approximately 75 of serum antibodies in humans igg is the most common type of antibody found in blood circulation. Further tests have also shown that this reagent is also suitable for use as a negative control with bovine maslanka et al 2012 ovine porcine kapetanovic et al 2012 equine jacks et al 2007 canine maiolini et al 2012 lapine pakandl et al 2008 and guinea pig tissues. Isotype controls should be matched to the primary antibody s host species and class including light chains e g igg2 lambda iga1 kappa. Mouse igg1 negative control is negative by flow cytometry on all human cells and cell lines tested.

This antibody is suitable for use as a non targeting isotype control in various in vitro and in vivo studies. Firstly it would confirm the specificity of your primary antibody. If using directly labeled primary antibodies the isotype control works best if conjugated with the same label as the test antibody. Detection of mouse igg 1 isotype control by flow cytometry human peripheral blood mononuclear cells pbmcs were stained with mouse anti human lilra6 cd85b monoclonal antibody catalog mab8656 filled histogram or mouse igg 1 isotype control antibody catalog mab002 open histogram followed by allophycocyanin conjugated anti mouse igg.

The human igg1 isotype control antibody is purified from human myeloma serum and is of unknown specificity. What experimental conditions should you consider. It can also be used as a negative control in various diagnostic applications such as elisa western blot immunofluorescence immunohistochemistry immunoprecipitation and flow. If using directly labeled primary antibodies it is important to use the same conjugate to avoid differences in detection.

Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. The isotype control antibody should be used in place of the primary antibody and with the same experimental conditions including. Secondly it will show you the fluorescence resulting from fc receptor mediated biding. If you are using a mouse igg1 monoclonal antibody that is conjugated to fitc you should select a mouse igg1 isotype control conjugated to fitc.

Selecting an isotype control. As the fluorophore conjugation to the antibody known as the f p ratio can vary between suppliers it is best to purchase the isotype from the same supplier as the primary. For example if you are using a mouse igg1 monoclonal antibody pe conjugated you should use a mouse igg1 pe conjugated isotype control. The rea control s antibody clone rea293 is a universal isotype control that can be used with all recombinant engineered antibodies reafinity antibodies that recognize cell surface antigens.

Although reafinity antibodies show virtually no binding to fc receptors the.